Polyclonal antibodies have been widely utilized in the area of detection of pathogens. Sheep are commonly used for the generation of polyclonal antibodies, due to their convenience in handling immunization and bleeding. Polyclonal antibody production in sheep can be generated more quickly and relatively cheaply and save the amount of expertise or time in comparison with monoclonal antibody production.
A comprehensive protocol that we developed of polyclonal antibody production includes a customizable choice for antigen preparation, immunizing sheep, collecting sera, antiserum detection, and antibodies purification. The specific service package is as follows.
1. We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.
2. Injections are subcutaneous as emulsions in FCA or FIA.
Procedure
Procedure | Protocol day | Description |
Control sera collection | 0 | Pre-immune sera (~5 mL per sheep) |
Injection | 1 | 0.50 mg antigen in FCA, ID 20 sites |
1st booster | 14 | 0.50 mg antigen in FIA, ID 20 sites |
2nd booster | 28 | 0.50 mg antigen in FIA, ID 20 sites |
Serum collection | 42 | 5 mL sera per sheep |
3rd booster | 56 | 0.50 mg antigen in FIA, ID 20 sites |
Sera collection | 56 | 5 mL sera per sheep |
4th booster | 70 | 0.50 mg antigen in FIA, ID 20 sites |
5th booster | 84 | 0.50 mg antigen in FIA, ID 20 sites |
Serum collection | 84 | 5 mL sera per sheep |
6th booster | 98 | 0.50 mg antigen in FIA, ID 20 sites |
7th booster | 112 | 0.50 mg antigen in FIA, ID 20 sites |
Serum collection | 112 | 5 mL sera per sheep |
ELISA and shipping | 118 | ELISA titration; Verify disposition of sheeps; Plasmapheresis from one sheep (~1,000 mL sera) provided when requested by customer |
Deliverables
22 vials crude antibody sera
2 vials: 1–2 mL (1 per sheep) test aliquots pre-immune sera
2 vials: 5 mL (1 per sheep) per vial pre-immune sera
8 vials: 1–2 mL (4 per sheep) test aliquots post-inject sera (4 times)
8 vials: 5 mL per vial post-inject sera (4 times)
1 vial: 1–2 mL test aliquot post-inject plasma (Plasmapheresis of one sheep)
1 vial: 1,000 mL bulk post-inject plasma (Plasmapheresis of one sheep)
We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.
Injections are subcutaneous as emulsions in FCA or FIA.