Rats are commonly used in laboratory for the production of polyclonal antibodies. We aim to custom comprehensive services for customers containing peptide synthesis, antigen production,rat immunization, sera collection, and titer dectection.
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We provide polyclonal antibody services from peptide synthesis, antigen production, antibody purification, to labeling. The specific service package is as follows.
Procedure
Procedure | Day | Description |
control sera collection | 0 | Pre-immune bleed (~1 mL per rat) |
Injection | 1 | 100 μg antigen in FCA, SQ 4 sites |
1st booster | 21 | 50 μg antigen inFIA, SQ 4 sites |
2nd booster | 42 | 50 μg antigen inFIA, SQ 4 sites |
Test bleed | 50 | 1 mL per rat |
ELISA and shipping | 60 | ELISA titration of pre-immune and test bleed; |
3rd booster | Day 62 | 50 μg antigen inFIA, SQ 4 sites |
Instruction due date | Day 80 | Terminal bleed (2–4 mL per rat), verify disposition of rats |
Deliverables
6 vials crude antibody sera
2 vials: 1 mL test aliquots pre-immune sera
2 vials: 1 mL test bleeds post-inject sera
2 vials: 2–4 mL terminal bleed
We guarantee to reach a minimum rabbit antiserum titer of 1:50,000 as measured with our ELISA assay.
Injections are subcutaneous as emulsions in FCA or FIA.