Service Number£ºABL016
Polyclonal antibodies are heterogeneous and bind to several different antigen epitopes. Rabbits are the most commonly used laboratory animals for the production of polyclonal antibodies. The rabbit offers many advantages over other species, such as adequate body size, easy procedure for the collection of large blood samples, excellent responsiveness to a wide variety of antigens, presence of a single primary IgG isotype, and reavailability of secondary reagents. Polyclonal antibody production is essential in research activity and the schedules and methodologies used to immunize rabbits and produce polyclonal antibodies continue to vary depending on the immunogen, the adjuvant, and the end purpose.
If you cannot find an antibody from our extensive antibody catalog to suit your needs, then custom antibody service team may help. Abvigen custom antibody production service offers gene/peptide/protein production technologies to suit your needs at affordable prices. From antigen design through to custom antibody generation, our expert scientists and talented technical support team have got you covered all the way.
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Phase | Details | Timeline |
Antigen preparation | Protein, recombinant or purified Peptide Small molecules Nucleic acid | 1-2 weeks |
Animal Immunization | Control serum collection Injection Serum collection ELISA titer detection | 4-6 weeks |
Antibody Purification | Protein A purification Protein G purification Protein A/G purification Affinity purification IgG purification | 1-2 weeks |
Antibody Verification | WB positive ELISA >1:10,000 or 1:100,000 | |
Antibody Conjugation | Biotin Horseradish peroxidase (HRP) Alkaline peroxidase Fluorochrom | 1-2 weeks |
Custom rabbit antibodies¡ªoptional protocol immunizing rabbits.
Rabbit titer guarantee¡ªrabbit antiserum titer of 1:10,000 to 1:100,000 as measured with our ELISA assay
Full-service integration¡ªcomplete set of services for antigen preparation and antibody purification
ELISA diagnostic assay shows highly specific reactivity and very low background.