Introduction
Baculovirus-insect cell expression system is one of the widely used eukaryotic expression systems at present. The baculovirus¨Cinsect cell expression system offers multifaceted advantages that set it apart from other expression systems. One of the most noteworthy advantages is its capacity to accommodate large DNA molecules within the vector, which enables the production of high-molecular-weight proteins. And it can house multiple gene insertions, allowing for the concurrent expression of different proteins. It has strong post-translational modification (PTM) capabilities within this system, encompassing essential processes such as glycosylation and acetylation. Abvigen can leverage the baculovirus expression system to support the production of difficult-to-express proteins, such as kinases, viral targets, cell surface receptors, transporters, various enzymes, and membrane proteins.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmi | 2 weeks |
Preparation of recombinant Bacmid and high titer virus | Isolate recombinant bacmid DNA Obtain baculovirus Repeat the infection | 2 weeks |
Scale up expression and purification | Infect insect cells Purification of target protein | 1 week |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Advantage
Short lead time
Cost-effective, high expression level
Multiple expression systems
One-stop service: from gene to protein expression purification
Large capacity: provides protein at milligram to gram levels
In addition, we also provide a variety of optional peptide Synthesis and analysis, including
| E. Coli Expression | Yeast Expression |
| Mammalian Expression | Large-Scale Protein Production |
Online Inquiry
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