Introduction
More complex protein structures usually require particular post-translational modifications and co-factors to support their elaborate folding and enzymatic activity. E.coli expression system are unable to perform these modifications and lack essential eukaryotic co-factors. There appears to be a progressive increase in the application of mammalian cells for proteins production because of its highest level of protein processing, which is important for complete biological activity. The introduction of large-scale transient transfection has enabled the use of mammalian cells more attractive in terms of speed and ease of use, particularly for cell surface and secreted glycoproteins. Abvigen enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.
Description
Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Small scale expression | Transient transfect HEK293, CHO and other cells Detect expression products Optimization of transfection conditions | 1-2 weeks |
Scale up expression and purification | Scale up the culture cells and transfect Optimal purification method | 1-2 weeks |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Advantage
Rapid expression of functional protein or antibody
Customization: quantity, purity, endotoxin, and other services are optional
Modifications: isotope labeling, protein biotinylation, and other services
Expression scale-up
In addition, we also provide a variety of optional protein expression system, including
| E. Coli Expression | Baculovirus-Insect Expression |
| Large-Scale Protein Production |
Online Inquiry
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