Fluorescent particles are spherical particles that contain fluorescent molecules, also known as fluorophores. These molecules absorb light at one specific color (excitation wavelength) and then emit light at another color (emission wavelength). This process provides high contrast and visibility of the particles against their background, making them valuable tools in various applications, including flow cytometry, fluorescence microscopy, fluid dynamics and cell labeling.
Abvigen is dedicated to providing researchers with fluorescent particles of the highest quality and performance. To ensure minimal dye leaching and photobleaching, our particles are prepared either staining the particle core with a fluorescent dye or polymerizing a fluorophore in the presence of the particle core. Abvigen's fluorescent polymer microspheres are available in a wide range of particle sizes, colors, densities, and excitation/emission wavelengths. They can be stored at room temperature or dispersed in aqueous media without compromising their fluorescent properties.
Key Advantages of Abvigen Fluorescent Particles:
High Fluorescence Intensity and Particle Size Uniformity: Ensures consistent and reliable results.
Broad Size Range: Particles range from a few nanometers to several hundred micrometers.
Versatile Matrix Options: Abvigen offers a variety of particle types, including: Polystyrene, Silica, PMMA, PVC, PLGA, PVA, Magnetic Particles, and Time-Resolved Fluorescent Particles.
Surface Availability for Conjugation: Fluorophores are embedded within the bead matrix, leaving the particle surface fully available for adsorbing molecules of interest, such as COOH, NH2, Biotin, and Streptavidin.
Wide Range of Excitation and Emission Wavelengths: Accommodates diverse flow cytometry filter sets, including Red, Orange, Green, Blue, and many others.
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Fluorescent | Excitation | Emission |
Red | 620 nm | 680 nm |
Orange | 540 nm | 580 nm |
Green | 488 nm | 518 nm |
Blue | 400 nm | 450 nm |
TR | 360 nm | 615 nm |
Ex/Em: 488 nm/518 nm
Ex/Em: 620 nm/680 nm
Ex/Em: 360-435 nm/450 nm
Orange 1: Ex/Em: 360-425 nm/610 nm
Orange 2: Ex/Em: 525-543 nm/580 nm
Ex/Em: 360 nm/615 nm
Highlights
Monodisperse Particles
High particle size uniformity
High-intensity fluorescence
Available with red, green orange and blue fluorescence
Designed with carboxylic acid groups (COOH) and amino groups (NH2) on the particle surface for the covalent binding of proteins, antibodies or other molecules
Offered with streptavidin on the surface for binding of biotinylated molecules
Outstanding long-term stability under proper storage conditions (both color and fluorescence)
Spherical shape
Great resistance to photobleaching
Minimized dye leaching
Suitable for uses in flow cytometry, fluorescence microscopy, phagocytosis studies and cell labeling
