Introduction
The baculovirus expression vector system (BEVS) is one of the eukaryotic expression systems commonly used in scientific research and industry. It belongs to the eukaryotic expression system and is easy to screen. The protein can be folded and modified to express the foreign gene after protein translation. It is highly efficient and is suitable for expressing proteins that may have a negative effect on mammalian host cells, such as kinases and toxic proteins. Therefore, BEVS has become a common technology for large-scale recombinant proteins in scientific research and industrial production.
Abvigen Inc has rich experience in the expression of recombinant proteins by insect-baculovirus. In addition, Abvigen Inc's genetic optimization services, protein design analysis services, flexible and quality service, and expression proposals can save customers a lot of time and money.
Description
1 Gene synthesis and codon optimization (optional)
2 Vector construction
Choose the expression system including expression vector, expression cells (Sf9, Sf21, Hi-5), transfection reagent according to customers
Subcloning to eukaryotic expression vector
Plasmid sequencing
Large amount of plasmid preparation
Cycle: 1-2 weeks
3 Virus packaging and amplification
Cycle: 2-3 weeks
4 Expression and purification
Virus infecting cells
Optimize expression and purification conditions
Recombinant protein identification
Cycle: 2-3 weeks
5 Large amount of expression and purification (optional)
Cycle: 2-3 weeks
Advantages
Efficient expression of intracellular and extracellular proteins
A variety of insect animal cells Sf9, Sf21, Hi-5 are available
Large-scale protein expression production
One-stop service: gene design and optimization, vector construction, viral packaging, expression purification, data analysis and report
Ordering Information:
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If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014
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