Introduction
The mammalian cell expression system can express the natural structurally intact protein, and is a widely used expression system for human complex glycosylation protein. It is the optimal expression of active protein factors and various receptor proteins as the biological activity is close to the natural protein. The system is widely used in the biopharmaceutical field, and more than 60% of the pharmaceutical protein products are obtained from mammalian cell expression systems.
Abvigen Inc’s experienced technical team has established an advanced platform for mammalian expression and purification technology. We can customize mg-to-g recombinant proteins with purity up to 95% and provide customers with flexible and quality services.
Description
1 Gene synthesis and codon optimization (optional)
2 Vector construction
Choose the expression system: expression vector, expression cells, transfection reagent
Subcloning to eukaryotic expression vector
Plasmid sequencing
Large amount of plasmid preparation
Cycle: 1-2 weeks
3 Transient transfection
Cell transient transfection
Test
Expression analysis and identification: WB
4 Expression and purification
Optimal conditions
Affinity purification
Recombinant protein identification
Cycle: 1-2 weeks
5 Large amount of expression and purification (optional)
Cycle: 2-3 weeks
Advantages
High expression level
Large-scale protein expression production
A variety of purification methods to meet customer demand for different purity recombinant proteins: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc.
One-stop service: gene design and optimization, transient transfection, stable cell line construction, data analysis and report processing
Ordering Information:
USA Europe Asia
Email: info@abvigenus.com Email: info@abvigenus.com Email: biotyscience@163.com
Tel: 1-816-388- 0112 Tel: +44 (0)20 8530 7621 Tel: +86 136 8125 6816
To learn more about information protection, please see Privacy Policy.
If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014
Online Inquiry