Introduction
Prokaryotic expression is mature and easy to operate. But for eukaryotes' proteins, misfolding often occurs due to glycosylation, folding partners, etc. The yeast expression system is the most cost-effective eukaryotic protein expression system, which can successfully achieve intracellular expression or secretory expression with its relatively inexpensive amplification medium, easier culture conditions, suitable for industrial amplification, and effectively reducing production costs. It is a powerful tool for expressing large-scale proteins. Yeast protein expression can be used to prepare protein products with high added value close to native proteins.
Abvigen Inc's yeast protein expression system (S. cescerevisiae and P. pastoris) is an important component of the recombinant protein expression platform. Abvigen Inc can provide gene sequence optimization, multi-vector selection construction, multi-strain transformation, large-scale production, and provide customer with high-purity soluble protein.
Description
1 Gene synthesis and codon optimization (optional)
2 Vector construction
Sequence analysis according to customer needs
Cloning into the expression vector
Plasmid sequencing
Large amount of plasmid preparation
Cycle: 2-3 weeks
3 Vector linearization and transformation
Select appropriate endonuclease for linearizing recombinant plasmid
Transferred to a suitable yeast cell by electroporation
Screen for high copy expression strains by PCR
Screen positive clones for test
Cycle: 1-2 weeks
4 Expression and purification
Optimize strain culture conditions
SDS-PAGE/WB
Cycle: 1-2 weeks
5 Large amount of expression and purification (optional)
Cycle: 2-3 weeks
6 Protein post processing (optional)
Label excision, endotoxin removal, lyophilization, N-terminal sequencing, etc.
Advantages
Efficient expression vector, high expression level: S. cescerevisiae and P. pastoris expression system for high success rate of protein expression
One-stop service: gene design and optimization, optimization of protein expression conditions, construction of constitutive expression strains, protein purification and modification, data analysis and report processing
Large-scale protein expression production
Ordering Information:
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If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014
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