A mouse monoclonal antibody is a type of antibody produced by a single clone of immune cells derived from mice. After immunization, antibody-secreting B cells are immortalized by fusion to myeloma cell lines, and the resultant hybridomas are screened using a client-directed customizable protocol to select for the desired antigen-specific antibody. Mouse immunization and spleen fusion to create hybridoma cell lines are two phases of an entire procedure for developing, producing, and purifying monoclonal antibodies.
Abvigen's custom mouse monoclonal antibody development service provides a comprehensive solution for generating high-quality monoclonal antibodies against any target of interest. The service includes immunogen design and synthesis, immunization, hybridoma generation, screening, and antibody production.
We perform and report in-process ELISA testing and send samples to customers for evaluation, allowing customers to select which animals to use for fusion and which cell lines to subclone, expand, and deliver.
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Phase | Details | Timeline |
Antigen preparation | Antigen designn Antigen synthesis and expression | Abvigen provided: 1-2 business days Peptide synthesis: 2-3 weeks Protein Expression: 2-4 weeks |
Immunization and ELISA titration | Conventional or accelerated immunization protocol ELISA Titer testing | 9-10 weeks |
Fusion and screening | Fusion clone screening by indirect ELISA Addition screening assays available upon request | 4-5 weeks |
Subcloning before production | Subclone screening | 6-7 weeks |
Production and purification | Small-scale antibody purification Isotype identification Additional screening and characterization assays available upon request Antibody purification Antibody validation by indirect ELISA Additional validation, scale-up, and antibody engineering available upon request Low-endotoxin and serum-free culturing are available on request, as well as high-volume production | 5-6 weeks |
Deliverables | 2-5 ELISA positive clones Purified antibodies, 0.1-0.5 mg/clone Remaining Antigens Final Project Report | |
Labeling | We offer a wide variety of Custom Antibody Labeling Services options | 1-2 weeks |
*Immunogen type: protein, peptides, small molecules, DNA, mRNA, virus-like particles, cell lines and etc.
Advanced antibody discovery platforms
Cutting-Edge Technology & Facility
Well-established generation platforms
Various Antibody Production Technologies
A wide range of antigens
Antibody validation approaches
Expert-guided custom project design