Introduction
The E. coli expression system is the classical protein expression system. It is the earliest, most widely used and most economical system in gene expression technology. This system has many advantages: clear cytogenetic background, high expression level of target gene, short culture period, convenient purification, good stability, strong anti-pollution ability, easy to enlarge and suitable for expression of various genera proteins.
Abvigen Inc's E. coli Protein Expression Service provides a full range of recombinant protein production services, with rich experience and expertise in the expression of soluble proteins, inclusion bodies, fusion proteins, protein purification and renaturation. In addition, we have a variety of E. coli cell lines and expression vectors to provide you with excellent prokaryotic protein expression services.
Description
1. Gene synthesis and codon optimization (optional)
The protein expression can be increased to several times after the gene sequence optimized by codon.
2. Vector construction
Provide correct plasmids with construction of fusion tag vectors including IPTG, arabinose, heat shock, cold shock induction, GST, Trx, SUMO, BMP, His, HA, Flag, myc, etc.
3. Expression strain screening
The expression plasmid was transformed into E. coli expression strain efficiently. SDS-PAGE electrophoresis was performed to select a suitable monoclonal strain for expression of the target protein.
Expression strains may be provided: DH5¦Á, Top10, BL21 (DE3), BL21 (DE3) pLys, Golden, Rosetta (DE3), RosettaBlue (DE3), Rosetta gami B (DE3), C41.
An expression strain containing recombinant plasmid is provided to the client.
4. Protein expression and purification
The target protein is purified by various chromatographic methods such as affinity chromatography, ion exchange, hydrophobic and gel filtration.
The label can be excised according to the requirements. Purify to obtain the target protein.
Providing the purified target protein to the customer. The final protein containing the purification tag or the excision purification tag can be supplied on request. The purity can be more than 95% or even higher according to the customer's requirements.
5. Inclusion body renaturation
Hundreds of buffer conditions for rapid screening of optimal refolding buffer, using dilution renaturation, dialysis renaturation, column chromatography renaturation and other techniques to obtain inclusion bodies with the purity greater than 90%.
6. Large-scale preparation of protein
We provide mature fermentation platform for high-density fermentation of E. coli or yeast.
Advantages
A variety of host and plasmid systems;
A variety of protein purification and protein refolding techniques to ensure correct protein folding and high protein solubility;
High-throughput protein expression levels;
One-stop services from gene synthesis, vector construction to purified protein;
Communicate service progress at any time;
Ordering Information
| USA | Europe | Asia | |
 | info@abvigenus.com | info@abvigenus.com | lfbioscience@163.com |
 | 1-816-388- 0112 | +44 (0)20 8530 7621 | +86 136 8125 6816 |
Online Inquiry
To learn more about information protection, please see Privacy Policy. If you have any questions about CRO service, please email at info@abvigenus.com or call 929-202-3014