Service Number：ABL030

Immunometric assay, also known as sandwich ELISA (enzyme-linked immunosorbent assay) is a powerful method for detecting and quantifying specific proteins. ELISA typically requires that the antigen of interest be captured or immobilized on a solid surface and then be complexed with an antibody that is linked to an enzyme. Immunometric assays exhibit a direct correlation between antigen concentration and substrate response. The first is what ELISA format to use—direct, indirect, or sandwich. Immunometric assays typically employ a "capture” antibody coated on the plate to bind the antigen of interest. Direct and indirect ELISA both immobilize the antigen on the assay plate and then use either a labeled primary antibody (direct) or primary antibody and labeled secondary antibody (indirect) to detect the antigen.  

Abvigen provides contract ELISA development kit services for the R&D and IVD community. Each Abvigen ELISA Development Kit contains the Capture Antibody, a biotinylated Detection Antibody, a calibrated antigen Standard, the Avidin-HRP conjugate, and a kit-specific protocol. We conduct ELISA kit development services for supporting regulatory approval submission. Each enzyme-linked immunosorbent assay is put through rigorous testing to ensure high precision, accuracy, sensitivity, and specificity. 

Optimization: an existing kit by testing other antibodies or reagents based on your special request.

Specific: ELISA assays features low reactivity with structurally related molecules.

Sensitive: better quantification thresholds measure low endogenous levels with ease.

Flexible: Our ELISA are adaptable to automated equipment for higher throughput systems.

Functional: Our ELISA Kits are validated in multiple sample matrices from serum and saliva to urine and feces.

Recovery and linearity: accurately detect the concentration of analyte in various types of sample matrices.

High precision and reproducibility.