Service Number：ABL029
Paired antibodies are two different antibodies generated against the same target, but with different binding regions or epitopes. Differential epitope recognition allows for simultaneous binding of both antibodies to the same target, making Sandwich ELISAs possible. This assay is a powerful tool used to quantify target analyte from complex samples across a myriad of fields. One antibody acts to capture the target and the other acts to detect it. They are applied in sandwich ELISA, IP-WB, LF, TIA, and protein-protein interaction.
Selection of antibodies use the traditional methods based on the immunization of animal. Whether generating a monoclonal antibody pair, a polyclonal antibody pair, or a combination, Abvigen's years of expertise in developing antibody pairs is at your disposal.
 |   |  |
 |  |
Phase | Details | Timeline |
Antigen generation | Gene synthesis and expression Peptide synthesis and coupling | 2-4 weeks |
Antibody generation | airing Formats: pAb/pAb; pAb/mAb; mAb/mAb Primary screening and sequencing Purification and labeling | 8-12 weeks |
Sandwich ELISA testing | Sandwich ELISA screen, all against all Final titration ELISA | 4-6 weeks |
Deliverables | Pre-immune serum Protein Antigen Antigen Affinity Purified Unlabeled Antibody Antigen Affinity Purified Biotinylated Antibody Hybridoma cell lines (mAb only) Validated sandwich ELISA repor | 1 week |
Batch-to-batch consistency
High specificity to samples
High Sensitivity
More flexibility in terms of conjugation and assay setup.