Service Number£ºABL024
Antibodies are indispensable research tools that have enabled researchers across diverse scientific fields to specify, identify, locate and/or quantify their protein targets. Polyclonal antibody production is essential in research activity and the schedules and methodologies used to immunize rabbits and produce polyclonal antibodies continue to vary depending on the animals and the end purpose. Unlike monoclonal antibodies, polyclonal antibodies correspond to multiple epitopes and tend to contain several immunoglobulin subtypes. They can be used as both primary and secondary antibodies in Westerns, ELISAs, and IHCs. Usually, rabbits, rats, goats, chickens, and guinea pigs are commonly used for the preparation of polyantibodies.
Antibodies raised in guinea pig can be used with antibodies raised in mouse, rabbit, goat, etc. in complex immunostaining or immunoprecipitation protocols. Abvigen brings back the classical guinea pig immunization and the novel monoclonal guinea pig antibody production service through our advanced phage display platform.
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Phase | Details | Timeline |
Antigen preparation | Peptide design, synthesis, conjugation Protein expression & purification Other antigen | 2-4 weeks |
Immunization & ELISA titration | Conventional or accelerated immunization protocol ELISA Titer testing | 8-10 weeks |
Antibody Purification | Protein A purification Protein G purification Protein A/G purification Affinity purification Antigen specific affinity purification | 1-2 weeks |
Antibody Verification | WB positive ELISA titer Optional: Application Validation Test, IHC, IF, F | 1-2 weeks |
Full-service integration¡ªcombine this immunization service with our complete set of services for antigen preparation and antibody purification.
Low price with fast turnaround: 3-5mg affinity purified antibody, in as fast as 80 days!
One-Stop Services: From antigen design, peptide synthesis or protein production to antibody production services.
Mature technology system - mature technology system and high experimental success rate.