Service Number£şABL002
Yeasts are outstanding hosts for the production of functional recombinant proteins with industrial or medical applications, and suitable for large-scale production of recombinant eukaryotic proteins. Yeast expression system provides the Highlights on production of secreted & intracellular targets and post-translational modifications, which may be beneficial for some recombinant proteins. The common yeast expression hosts used for recombinant proteins expression include Saccharomyces cerevisiae, Pichia pastoris, Hansenula polymorpha, Yarrowia lipolytica, Arxula adeninivorans, Kluyveromyces lactis, and Schizosaccharomyces pombe. In Abvigen, various host cells and vectors are available for yeast expression.
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| Phase | Procedure | Timeline |
| Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
| Transformation and strain screening | Linearization of recombinant plasmid Transform hosts by electroporation Verify successful transformants by PCR Obtain high copy | 1-2 weeks |
| Small test | Small scale expression screening | 2 weeks |
| Scale up expression and purification | Optimize expression conditions Scale up culture Protein purification | |
| Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 weeks |
Proprietary Technologies: promoters, optimal copies of vector per cell, inducibility, signal peptides, and cellular location.
Humanized antibody production in yeast expression system.
Streamlined Services: from gene synthesis, to the protein expression, antibody production, et al.