Introduction
Endotoxins, known as lipoglycans and lipopolysaccharides (LPS), are a part of the outer membrane of the cell wall of Gram-negative bacteria. It is extremely hard to remove them by simple sterilization. Endotoxins can initiate a strong immune response such as endotoxin shock, organ failure, tissue injury, et al. Due to the harmful effects of endotoxin, limiting the protein endotoxin level has become an important direction in the medical field. E. Coli is a preferred system for recombinant protein because it generally provides the most cost effective solution. However, there are several limitations, include difficultly in production for proteins with strict endotoxin condition requirements and poor secretion for secretory proteins. To break out the limit, Abvigen has developed endotoxin-free Expression System, which is able to express a wide variety of protein and oligonucleotides as the wild-type counterparts except that they lack endotoxic activity.
Description
Phase | Procedure |
Gene synthesis | Codon optimization; gene synthesis |
Protein expression & purification | Transformation and strain screening Target protein expression Protein purification |
Endotoxin removal | Endotoxin affinity chromatography Ion exchange chromatography Hydrophobic chromatography Gel filtration chromatography and ultrafiltration Triton X-114 phase separation |
Endotoxin detection | LAL gel-clot assay LAL turbidimetric assay LAL chromogenic assay |
In addition, we also provide a variety of optional peptide Synthesis and analysis, including
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