Service Number£ºABL018
Overview
Nowadays, antibody drugs are increasingly important for the pharmaceutical industry across the globe. In contrast to polyclonal antibodies, a monoclonal antibody is derived from a single B cell parent clone and will only recognize a single epitope per antigen. With the development of antibody preparation techniques, single B cell isolation offers a fast, high-throughput method to generate a cell line producing your antibody of interest.
B cells are isolated from an immunized animal, sorted into single wells, lysed, and their genomes sequenced. Genes encoding desired proteins are cloned into a vector and transiently transfected into mammalian cells. Single B cell antibody production has many advantages such as short turnaround time, low hands-on inputs, and simple operation process and few rounds of cell culture. Abvigen offers a high-throughput,full integrated platform for accelerating antibody discovery. And the method allows detection of those very rare antibodies orpotential drugs with unique properties that are exceptionally hard to find.
Phase | Details | Timeline |
Antigen Preparation | Protein, recombinant or purified Peptide Small molecules Nucleic acid | 1-2 weeks |
Immunization | 5-10 BALB/c mouse DNA, Protein, Cells 1-2 months, maintain repertoire diversity | 4-6 weeks |
Plasma B Cells Isolation | Isolate plasma B cells from Spleen and Bone Marrow | 1-2 weeks |
Single B Cell Screening | Up to 2E6 plasma B cells are screened. Protein binding Cell-based binding Sorting positive micro-droplets | 4-5 weeks |
Single Cell Sequencing | Cell sequencing High throughput expression | 1-2 weeks |
Validation | Evaluate product quality ELISA titer detection | 1-2 weeks |
Antibodies directly from primary plasma cells
High-throughput screening of B cells
Available for most species
Fast turnaround time
Identify targets directly from B cells
More Chance to ldentify Rare Antibodies