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In addition to gene therapy protocols, improved non-viral gene delivery techniques are also of significant interest in transient gene expression in cell culture, especially in suspension culture. Such a transient transfection system has many Highlightss. It would speed up the process of expressing milligram amounts of recombinant proteins, and be used to transfect different cell lines simultaneously to obtain sufficient amounts of ‘test’ protein. In order to establish transient gene expression in mammalian cells grown in suspension culture, more strict culture conditions are required. Serum-free media are media designed to grow a specific cell type or perform a specific application in the absence of serum. The use of serum-free media (SFM) represents an important tool, that allows cell culture to be done with a defined set of conditions as free as possible of confounding variables. Abvigen has applied Serum-free technology for production of monoclonal antibodies, viral antigens, and recombinant proteins using a variety of mammalian and invertebrate cell lines.

Allows the cultivation and expansion of cells under serum-free conditions. 

Fully defined in its composition.

Experimental conditions constant and comparable.

Highly reproducible.

High proliferation rate.

Largely retain their undifferentiated state.