Service Number£şABL004
More complex protein structures usually require particular post-translational modifications and co-factors to support their elaborate folding and enzymatic activity. E.coli expression system are unable to perform these modifications and lack essential eukaryotic co-factors. There appears to be a progressive increase in the application of mammalian cells for proteins production because of its highest level of protein processing, which is important for complete biological activity. The Overview of large-scale transient transfection has enabled the use of mammalian cells more attractive in terms of speed and ease of use, particularly for cell surface and secreted glycoproteins. Abvigen enables the high-throughput delivery and the generation high-quality of recombinant proteins and antibodies that are widely used in applications such as antigen preparation, antibody discovery, protein structure elucidation, and research for protein function.
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Phase | Procedure | Timeline |
Plasmid construction | Codon optimization; gene synthesis Ligation to expression vector Obtain the correct recombinant plasmid | 2 weeks |
Small scale expression | Transient transfect HEK293, CHO and other cells Detect expression products Optimization of transfection conditions | 1-2 weeks |
Scale up expression and purification | Scale up the culture cells and transfect Optimal purification method | 1-2 weeks |
Quality Control | Testing of purity, concentration, etc. QC report is provided. | 1 week |
Rapid expression of functional protein or antibody.
Customization: quantity, purity, endotoxin, and other services are optional.
Modifications: isotope labeling, protein biotinylation, and other services.
Expression scale-up.