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Mammalian Protein Expression Services

Introduction

 

The mammalian cell expression system can express the natural structurally intact protein, and is a widely used expression system for human complex glycosylation protein. It is the optimal expression of active protein factors and various receptor proteins as the biological activity is close to the natural protein. The system is widely used in the biopharmaceutical field, and more than 60% of the pharmaceutical protein products are obtained from mammalian cell expression systems.

 

Abvigen Inc¡¯s experienced technical team has established an advanced platform for mammalian expression and purification technology. We can customize mg-to-g recombinant proteins with purity up to 95% and provide customers with flexible and quality services.

 

Description

 

1 Gene synthesis and codon optimization (optional)

 

2 Vector construction

 

Choose the expression system: expression vector, expression cells, transfection reagent

 

Subcloning to eukaryotic expression vector

Plasmid sequencing

Large amount of plasmid preparation

 

Cycle: 1-2 weeks

 

3 Transient transfection

 

Cell transient transfection

 

Test

 

Expression analysis and identification: WB

 

4 Expression and purification

 

Optimal conditions

 

Affinity purification

 

Recombinant protein identification

 

Cycle: 1-2 weeks

 

5 Large amount of expression and purification (optional)

 

Cycle: 2-3 weeks

 

Advantages

 

High expression level

 

Large-scale protein expression production

 

A variety of purification methods to meet customer demand for different purity recombinant proteins: affinity chromatography, ion exchange, hydrophobic chromatography, gel filtration, etc.


One-stop service: gene design and optimization, transient transfection, stable cell line construction, data analysis and report processing

 

Ordering Information:


USA                                                  Europe                                                   Asia

 

Email: info@abvigenus.com                   Email: info@abvigenus.com                             Email: biotyscience@163.com

 

Tel: 1-816-388- 0112                                  Tel: +44 (0)20 8530 7621                                  Tel: +86 136 8125 6816

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