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Insect Protein Expression Services

Introduction

 

The baculovirus expression vector system (BEVS) is one of the eukaryotic expression systems commonly used in scientific research and industry. It belongs to the eukaryotic expression system and is easy to screen. The protein can be folded and modified to express the foreign gene after protein translation. It is highly efficient and is suitable for expressing proteins that may have a negative effect on mammalian host cells, such as kinases and toxic proteins. Therefore, BEVS has become a common technology for large-scale recombinant proteins in scientific research and industrial production.

 

Abvigen Inc has rich experience in the expression of recombinant proteins by insect-baculovirus. In addition, Abvigen Inc's genetic optimization services, protein design analysis services, flexible and quality service, and expression proposals can save customers a lot of time and money.

 

Description

 

1 Gene synthesis and codon optimization (optional)

 

2 Vector construction

 

Choose the expression system including expression vector, expression cells (Sf9, Sf21, Hi-5), transfection reagent according to customers

 

Subcloning to eukaryotic expression vector

Plasmid sequencing

Large amount of plasmid preparation

Cycle: 1-2 weeks

 

3 Virus packaging and amplification

 

Cycle: 2-3 weeks

 

4 Expression and purification

 

Virus infecting cells

 

Optimize expression and purification conditions

 

Recombinant protein identification

 

Cycle: 2-3 weeks

 

5 Large amount of expression and purification (optional)

 

Cycle: 2-3 weeks

 

Advantages

 

Efficient expression of intracellular and extracellular proteins

 

A variety of insect animal cells Sf9, Sf21, Hi-5 are available

 

Large-scale protein expression production

 

One-stop service: gene design and optimization, vector construction, viral packaging, expression purification, data analysis and report


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